Methionyl Soluble Ribonucleic Acid Transformylase
نویسندگان
چکیده
منابع مشابه
Methionyl Soluble Ribonucleic Acid Transforrnylase
The MO-formyltetrahydrofolate:methionyl soluble ribonucleic acid transformylase has been purified over 1500-fold from Escherichia coli B. Although not homogeneous at this stage, the enzyme was of sufficient purity to determine some physical characteristics. The sedimentation constant was 2.02 which would indicate a molecular weight of approximately 25,000. In addition to the substrates of the r...
متن کاملRecognition of tRNAs by Methionyl-tRNA transformylase from mammalian mitochondria.
Protein synthesis involves two methionine-isoaccepting tRNAs, an initiator and an elongator. In eubacteria, mitochondria, and chloroplasts, the addition of a formyl group gives its full functional identity to initiator Met-tRNA(Met). In Escherichia coli, it has been shown that the specific action of methionyl-tRNA transformylase on Met-tRNA(f)(Met) mainly involves a set of nucleotides in the ac...
متن کاملSpecies Specificity of Amino Acid Acceptor Ribonucleic Acid and Aminoacyl Soluble Ribonucleic Acid Synthetases.
Several investigators have reported that the activation of amino acids for protein synthesis, i.e. the incorporation of amino acids into soluble ribonucleic acids, is dependent upon the source of both the aminoacyl S-RNA’ synthetases and the S-RNA itself. For example, Rendi and Ochoa (1) reported that aminoacyl S-RNA synthetases from Escherichia coli could not incorporate leucine into yeast or ...
متن کاملThe aminoacylation of transfer ribonucleic acid. Recognition of methionine by Escherichia coli methionyl-transfer ribonucleic acid synthetase.
The mechanism of the recognition of methionine by Escherichia coli methionyl-tRNA synthetase was examined by a kinetic study of the recognition of methionine analogues in the ATP-PPi exchange reaction and the tRNA-aminoacylation reaction. The results show that the recognition mechanism consists of three parts: (1) the recognition of the size, shape and chemical nature of the amino acid side cha...
متن کاملStudies on Soluble Ribonucleic Acid: the Action of Snake Venom Phosphodiesterase on Soluble Ribonucleic Acid in Yeast.
Useful information on the structure of soluble ribonucleic acid may be obtained by a study of the action of snake venom diesterase on it, to the extent that the mechanism of action of the enzyme itself is understood. Snake venom phosphodiesterase has been widely studied and extensively purified (l-5). Two basic conclusions have been reached as to its mechanism of action on ribonucleotide and de...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96123-5